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 Table of Contents  
REVIEW ARTICLES
Year : 2023  |  Volume : 9  |  Issue : 1  |  Page : 1-4

Staphylococcal carriage status: implications, mechanisms, and practical treatment guidelines


Department of Dermatology Venereology and Leprosy, All India Institute of Medical Sciences, Rishikesh, India

Date of Submission20-Oct-2022
Date of Acceptance09-Dec-2022
Date of Web Publication24-Aug-2023

Correspondence Address:
Atreyo Chakraborty
Department of Dermatology Venereology and Leprosy, All India Institute of Medical Sciences, 26 C Selimpore Lane, Rishikesh, Dhakuria, Kolkata 700031
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijdd.ijdd_26_22

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  Abstract 

Staphylococcal carriage status is very common, and it is important for the dermatologist to be aware of its clinical manifestations, the mechanisms by which they produce colonization, and the therapeutic options available to eliminate them.

Keywords: Carriage elimination, mupirocin, skin and soft tissue infection, staphylococcus carriage


How to cite this article:
Chakraborty A. Staphylococcal carriage status: implications, mechanisms, and practical treatment guidelines. Indian J Drugs Dermatol 2023;9:1-4

How to cite this URL:
Chakraborty A. Staphylococcal carriage status: implications, mechanisms, and practical treatment guidelines. Indian J Drugs Dermatol [serial online] 2023 [cited 2024 Feb 24];9:1-4. Available from: https://www.ijdd.in/text.asp?2023/9/1/1/384289




  Introduction Top


Staphylococcus aureus is an important commensal of the human skin and an important constituent of the cutaneous microbiome. While it has been implicated in a number of conditions such as recurrent furunculosis, abscesses, staphylococcal scalded skin syndrome, and so on, it remains dormant in a large number of populations viz hospital workers, sanitation workers, the immunocompromised and so on. When they greatly exceed their presence in the normal skin, they often cause symptoms.


  Factors Allowing Staph Aureus to Adapt to the Skin Microbiota Top


By adapting itself to the local skin milieu, Staph aureus has become a successful member of the cutaneous microbiota.[1],[2] However, for any bacterium (note: the singular of bacteria is bacterium) to successfully adapt to host conditions, the following conditions, must be fulfilled:

  • A. Staph aureus possesses a multitude of virulence factors for example ET A, B, and many more that allow it to quickly lyze the cell and cause havoc to the immune system. However, if such virulence was allowed to continue, Staph aureus would have been quickly detected by the host immune response and destroyed. Hence, to avoid detection, Staph aureus tightly downregulates its virulence during its stay as commensal.


  • B. It develops an adhesion mechanism. There is evidence that adhesion to cell membranes is tighter in Staph aureus colonizing the skin than in pathogenic virulent ones. The tighter adherence ensures it is not rubbed off the skin regularly during washing/itching.


  • C. In a similar way, gene products that enhance colonization such as defense against reactive oxygen species (remember nose is exposed to a lot of oxygen), evasion of the immune response, and so on are upregulated.



  Colonization Risk Factors Top


There are many risk factors postulated for Staphylococcal carriage status and these include living in a hot and humid environment, healthcare occupation, having anemia or other hematological deficiencies, and metabolic diseases such as diabetes, gout, and blood dyscrasias.


  Colonization Sites Top


Various sites have been reported to have been colonized by Staph aureus and they include:[3],[4] the squamous epithelium on the nasal septum adjacent to the nasal ostium, axillae (8%), chest and abdomen (15%), the perineum (22%), the intestines (17–33%), vagina (5%) and the pharyngeal wall (4–64%).[3],[4]


  Types of Carriage Top


Based on the methods of sampling, three types of carriage have been detected: persistent, intermittent, and noncarriage.[4]

Persistent is defined as two or more cultures performed at 1 week apart and both are positive. Intermittent is defined as only one out of two cultures performed at 1 week apart and is positive. When both cultures are negative it is termed as noncarriage. It is well known that persistent carriers have a higher bacterial load than nonpersistent carriers.


  Duration of Carriage Top


The duration of carriage is 4–14 days postinoculation for intermittent and noncarriers whereas it is on average 154 days for persistent carriers.[5]


  Determinants of Bacterial Carriage Top


There are many factors both from the host site and from the bacteria side which determine whether successful bacterial colonization will occur or not. They are:

  • A. Bacterial determinants
    • i) Expression of adhesion molecules sdr C. sdr D, sdr E. In general, the skin is exposed to a lot of rubbing and scrubbing which in a normal setting usually dislodges the bacteria. These adhesion molecules ensure tight adhesion to the cell membranes, particularly of the skin.


    • ii) Clumping proteins clf A, clf B, fnb A, fnb B: They also help in the formation of clumping of RBCs around them to protect them from further attacks of leukocytes.


    • iii) Biofilm formation by binding of clf B to CK 8 and 10 on keratinocytes: This phenomenon is one of the most important strategies to evade the host immune response. A biofilm is a collection of bacterial slime, bacteria, and host keratinocyte sloughs which results in a tough membrane, restricting the access of antibiotics and host immune cells. In fact, biofilm formation is now implicated for many cases of emerging bacterial resistance.


    • iv) Other bacterial flora: Corynebacteria on the nose and strep pneumonia significantly reduce Staph carriage.


    • v) Quorum sensing: Staphylococcus can self-regulate their number on many occasions—if the number is too great, the cells signal to other cells to stop multiplying and in this way, the population is kept in check. This mechanism might explain why staphylococcus has been so successful in colonization our skin because any excess bacteria would be detected by the immune cells and rapidly killed.


  • B. Host determinants[6]

    • i) Toll-like receptor polymorphisms: Toll-like receptors (TLR) are a part of the natural immune system that is innate. Their signaling is mediated through protein receptors on the surface that look like lamp posts, hence the name Toll. They detect molecular patterns that are common to all pathogens and thereby mount an immune system in a nonspecific manner. There are around 20 types of Toll like receptor (TLR) discovered so far and each has specific functions—for example, TLR 2 works with gram-negative organisms and has been implicated in acne. TLR 4 deals with gram-positive organisms and their polymorphism means they do not work the same way in everyone—hence such polymorphism can enhance susceptibility to Staphylococcal carriage


    • ii) Defensin and Cathlicidin polymorphism: They are natural antipathogenic molecules, which are reduced in some conditions such as atopic dermatitis. Their deficiency can account for increased staphylococcal carriage in these conditions as well as host carriage.


    • iii) Mutations in glucocorticoid receptor genes: A 80% reduced carriage is seen with those with a particular mutation in Glucocorticoid receptors (GCR).


    • iv) Reduced Vit D levels.


    • v) Chronic Inflammation—viz atopic eczema.





  The Risks of Staphylococcal Carriage Status Top


The carriage has been identified as an important risk factor for serious infections in these patients: Those undergoing surgery; those on hemodialysis or Continuous ambulatory peritoneal dialysis (CAPD); Those with HIV infection and AIDS; those with intravascular devices and those colonized with Methicillin-resistant Staphylococcus aureus (MRSA).[7]

Elimination of carriage has been found to reduce the infection rates in surgical patients and those on hemodialysis and CAPD. Elimination of carriage appears to be an attractive preventive strategy in patients at risk.

  • A. Staphylococcal niche sites: These are the sites where staphylococcus is most often found and include:
    • i) Nose: The anterior nares, at the junction where the bony nasal septum meets the mucosa.


    • ii) Axillae.


    • iii) Chest and abdomen, particularly periumbilical.


    • iv) Perineum.


    • v) Posterior pharyngeal mucosa.





  How to Eliminate Staphylococcal Carriage Top


Various drugs or antiseptics have been in use for the elimination of staphylococcal carriage. They are summarized in the following [Table 1].[8],[9],[10]
Table 1: Drug treatment of staphylococcal carriage

Click here to view



  Guidelines for Staphyloccal Elimination Carriage Top


Indications of Staphylococcal carriage elimination:

  • A. Recurrent SSTI around mouth and nose: These most often take the form of recurrent furunculosis and abscesses. Perinatal abscesses are also seen. Sometimes even cellulitis has been seen. The benefits of weekly pulses of mupirocin are well established—in a recent study, the relative incidence before and after mupirocin prophylaxis was 0.84 vs. 0.03, which was statistically significant. The regimen being very cheap and effective should be the first line for the management of recurrent skin and soft tissue infections around the nose.[11] The regimen was equally effective for community-acquired methicillin-resistant Staphylococcus aureus vs. methicillin-sensitive Staphylococcus aureus.


  • B. Before undertaking surgery of skin and soft tissues: This is associated with a reduced rate of postoperative infections. This has also been validated in major surgeries involving infected sites.[12] Many times dermatologists are called to advice and give clearance for surgery, often involving minor infections at the proposed sites—in such situations elimination of staphylococcus by either systemic or topical means is conducive to a better outcome.


  • C. Community-acquired pneumonia and bacteremia prevention in the elderly and other immunocompromised: Nasal elimination of staphylococcus can often be lifesaving for at-risk population, such as those on peritoneal hemodialysis—the incidences of pneumonia and other fatal infections are also reduced. The dermatologist can advise their colleagues regarding the same. Yearly or twice-yearly prophylaxis is probably enough to reduce carriage status; however, formal studies are needed.[13]


  • D. Predictor of pneumonia in postburn patients: Staphylococcus carriage status is one of the best predictors of pneumonia and bacteremia in postburn patients. Elimination of carriage status might help improving survival among these patients.[14]


  • E. Severe recalcitrant hand eczema: The evidence that in noninfected hand eczema, elimination of Staphylococcal carriage results in improvement is at best equivocal. It is mostly derived from the coincident elimination of hand Staphylococcal aureus when mupirocin is also applied in the nasal nares. In many cases, improvement is also seen, coincident with such elimination.[15]



  High-Level Mupirocin Resistance Top


High-level, plasmid-mediated mupirocin resistance has been documented in some nations, warranting the necessity of the other regimens listed above.[16] Since mupirocin resistance is mediated via the mupA gene, which distorts the isoleucine t-RNA synthase, related groups of drugs are also incapable to clear such organisms. In such circumstances, other options listed above should be tried. However, the real clinical implications of such resistance are still unknown.[17]


  Conclusion Top


The dermatologist should familiarize themselves with the various modalities available to eradicate staphylococcus from the skin, which often translates to a reduction in systemic and skin and soft tissue infections. Being simple, cost-effective and of a relatively shorter duration, such prophylaxis should be given wherever indicated.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Vandenesch F, Lina G, Henry T Staphylococcus aureus hemolysins, bi-component leukocidins, and cytolytic peptides: A redundant arsenal of membrane-damaging virulence factors? Front Cell Infect Microbiol 2012;2:12.  Back to cited text no. 1
    
2.
Coutts CG, Jonsson IM, Tarkowski A, Kokai-Kun JF, Mond JJ, Foster SJ Catalase (KatA) and alkyl hydroperoxide reductase (AhpC) have compensatory roles in peroxide stress resistance and are required for survival, persistence, and nasal colonization in Staphylococcus aureus. J Bacteriol 2007;187:1025-35.  Back to cited text no. 2
    
3.
Cole AM, Tahk S, Oren A, Yoshioka D, Kim YH, Park A, et al. Determinants of Staphylococcus aureus nasal carriage. Clin Diagn Lab Immunol 2001;8:1064.  Back to cited text no. 3
    
4.
Williams E Healthy carriage of Staphylococcus aureus: Its prevalence and importance. Bacteriol Rev 1963;27:56-71.  Back to cited text no. 4
    
5.
Verkaik VBNJ, Devogel CP, Boelens HA, Verveer J, Nouwen JL, Verbrugh HA, et al. Reclassification of Staphylococcus aureus nasal carriage types. J Infect Dis 2009;199:1820-6.  Back to cited text no. 5
    
6.
Youssef D, Bailey B, El Abbassi A, Copeland R, Adebonojo L, Manning T, et al. Healthcare costs of staphylococcus aureus and clostridium difficile infections in veterans: Role of vitamin D deficiency. Epidemiol Infect 2010;138:1322-7.  Back to cited text no. 6
    
7.
Kluytmans J, van Belkum A, Verbrugh H Nasal carriage of staphylococcus aureus: Epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev 1997;10:505-20.  Back to cited text no. 7
    
8.
Ammerlaan HSM, Kluytmans JAJW, Wertheim HFL, Nouwen JL, Bonten MJM Eradication of methicillin-resistant staphylococcus aureus carriage: A systematic review. Clin Infect Dis 2009;48:922-30.  Back to cited text no. 8
    
9.
Ghaddara HA, Kumar JA, Cadnum JL, Ng-Wong YK, Donskey CJ Efficacy of a povidone iodine preparation in reducing nasal methicillin-resistant Staphylococcus aureus in colonized patients. Am J Infect Control2020;48:456-9.  Back to cited text no. 9
    
10.
Gemmell CG, Edwards DI, Fraise AP, Kate Gould F, Ridgway GL, Warren RE; on behalf of the Joint Working Party of the British Society for Antimicrobial Chemotherapy, Hospital Infection Society and Infection Control Nurses Association. Guidelines for the prophylaxis and treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections in the UK. J Antimicrob Chemothe 2006;57:589-608,  Back to cited text no. 10
    
11.
Miller LG, Tan J, Eells SJ, Benitez E, Radner AB Prospective investigation of nasal mupirocin, hexachlorophene body wash, and systemic antibiotics for prevention of recurrent community-associated methicillin-resistant staphylococcus aureus infections. Antimicrob Agents Chemother 2012;56:1084-6.  Back to cited text no. 11
    
12.
Kluytmans J Reduction of surgical site infections in major surgery by elimination of nasal carriage of staphylococcus aureus. J Hosp Infect 1998;40 Suppl B:S25-9.  Back to cited text no. 12
    
13.
Boyce JM Preventing staphylococcal infections by eradicating nasal carriage of staphylococcus aureus: Proceeding with caution. Infect Control Hosp Epidemiol 1996;17:775-9.  Back to cited text no. 13
    
14.
Fournier A, Voirol P, Krähenbühl M, Bonnemain CL, Fournier C, Dupuis-Lozeron E, et al. Staphylococcus aureus carriage at admission predicts early-onset pneumonia after burn trauma. Eur J Clin Microbiol Infect Dis 2017;36:523-8.  Back to cited text no. 14
    
15.
Reagan DR, Doebbeling BN, Pfaller MA, Sheetz CT, Houston AK, Hollis RJ, et al. Elimination of coincident staphylococcus aureus nasal and hand carriage with intranasal application of mupirocin calcium ointment. Ann Intern Med 1991;114: 101-6.  Back to cited text no. 15
    
16.
Kizerwetter-Swida C-C, Rzeuska M High-level mupirocin resistance in methicillin resistance staphylococcus isolated from dogs and cats. BMC Vet Res 2019;238:2217.  Back to cited text no. 16
    
17.
Poovelikunnel T, Gethin G, Humphreys H Mupirocin resistance: Clinical implications and potential alternatives for the eradication of Mrsa. J Antimicrob Chemother 2015;70:2681-92.  Back to cited text no. 17
    



 
 
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  In this article
Abstract
Introduction
Factors Allowing...
Colonization Ris...
Colonization Sites
Types of Carriage
Duration of Carriage
Determinants of ...
The Risks of Sta...
How to Eliminate...
Guidelines for S...
High-Level Mupir...
Conclusion
References
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